Journal: Biofactors (Oxford, England)

Article Title: Anticancer effect of minor phytocannabinoids in preclinical models of multiple myeloma

doi: 10.1002/biof.2078

Figure Lengend Snippet: CBG, CBC, CBN, and CBDV effect on HuOB cell line viability. HuOB were treated for 72 h and then MTT assay was used to determine cell viability. Three separate experiments are shown as mean ± SD. * p < 0.05, ** p < 0.01, **** p < 0.0001 versus VHC.

Article Snippet: Human osteoblast cell line CI‐huOB (HuOB) was purchased from InSCREENex GmbH (Braunschweig, Germany) and cultured in DMEM glucose high medium (EuroClone, Milan, Italy) supplemented with 100 IU/mL penicillin, 100 mg streptomycin, 10% FBS, 1 mM sodium pyruvate, and 2 mM L‐glutamine.

Techniques: MTT Assay

Journal: Biofactors (Oxford, England)

Article Title: Anticancer effect of minor phytocannabinoids in preclinical models of multiple myeloma

doi: 10.1002/biof.2078

Figure Lengend Snippet: MM cell invading the ECM covered membrane toward HuOB cells, after treatment with CBG, CBC, CBN, and CBDV. (A) Representative image of calcein stained RPMI cells invading the ECM coated membrane. Three random fields were observed under a fluorescence microscope. Magnification 10×. (B) The number of invading cells represent the mean ± SD of three separated experiments. * p < 0.05, ** p < 0.01, **** p < 0.0001 versus UNTREATED.

Article Snippet: Human osteoblast cell line CI‐huOB (HuOB) was purchased from InSCREENex GmbH (Braunschweig, Germany) and cultured in DMEM glucose high medium (EuroClone, Milan, Italy) supplemented with 100 IU/mL penicillin, 100 mg streptomycin, 10% FBS, 1 mM sodium pyruvate, and 2 mM L‐glutamine.

Techniques: Membrane, Staining, Fluorescence, Microscopy

Journal: International Journal of Biological Sciences

Article Title: Vitamin K 2 Ameliorates Damage of Blood Vessels by Glucocorticoid: a Potential Mechanism for Its Protective Effects in Glucocorticoid-induced Osteonecrosis of the Femoral Head in a Rat Model

doi: 10.7150/ijbs.15248

Figure Lengend Snippet: VEGF expression analysis in EAhy926 and MG63. A. Western blotting analysis of total cell lysates in EAhy926, β-actin was used as the loading control. B. VEGF mRNA expression in EAhy926 treated with or without DEX and VK 2 , each condition was performed in triplicate (* indicated P < 0.05; ** indicated P < 0.01). C. Western blotting analysis of total cell lysates in MG63. β-actin was used as the loading control. D. VEGF mRNA expression in MG63 cells treated with or without DEX and VK 2 , each condition was performed in triplicate (* indicated P < 0.05; ** indicated P < 0.01).

Article Snippet: The human endothelial cell line EAhy926 and the human osteoblast-like cell line MG63 were obtained from KeyGENE BIOTECH (Nanjing, China).

Techniques: Expressing, Western Blot, Control

Journal: International Journal of Biological Sciences

Article Title: Vitamin K 2 Ameliorates Damage of Blood Vessels by Glucocorticoid: a Potential Mechanism for Its Protective Effects in Glucocorticoid-induced Osteonecrosis of the Femoral Head in a Rat Model

doi: 10.7150/ijbs.15248

Figure Lengend Snippet: Effect of DEX and VK 2 on angiogenic factors in EAhy926 and MG63. (A-E) Quantitative RT-PCR analysis of KDR, Flt, vWF, CD31 and PDGF-B in EAhy926. (F,G) TGF-β and BMP-2 mRNA expression in MG63. (Each condition was performed in triplicate, * indicated P < 0.05, ** indicated P < 0.01).

Article Snippet: The human endothelial cell line EAhy926 and the human osteoblast-like cell line MG63 were obtained from KeyGENE BIOTECH (Nanjing, China).

Techniques: Quantitative RT-PCR, Expressing

Journal: Oncology Letters

Article Title: Computational discovery of novel colony-stimulating factor-1 receptor as a potential therapeutic biomarker in osteosarcoma and a novel inhibitor from herbal sources

doi: 10.3892/ol.2026.15618

Figure Lengend Snippet: Cell viability (%) of MG63 and Saos-2 cells treated with pexidartinib (via PLX3397) and sotuletinib.

Article Snippet: MG63 osteosarcoma cells were purchased from Procell Life Science Technology Co., Ltd. (cat. no. #CL-0157; http://www.procell.com.cn/p/mg-63-cl-0157-72575 ).

Techniques:

Journal: Oncology Letters

Article Title: Computational discovery of novel colony-stimulating factor-1 receptor as a potential therapeutic biomarker in osteosarcoma and a novel inhibitor from herbal sources

doi: 10.3892/ol.2026.15618

Figure Lengend Snippet: Sensitization of the MG63 and Saos-2 cell lines to a CSF1R inhibitor. (A) Dose-dependent cytotoxic effects of linarin on MG63 and Saos-2 osteosarcoma cell viability. (B) Dose-dependent cytotoxic effects of saikosaponin C on MG63 and Saos-2 osteosarcoma cell viability. (C) Dose-dependent cytotoxic effects of sarsasapogenin on MG63 and Saos-2 osteosarcoma cell viability. (D) Molecular docking of the binding between sarsasapogenin and the CSF1R protein. (E) Visual molecular docking of the binding acid residues between sarsasapogenin and CSF1R protein. (F) The expression levels of CSF1R as detected by western blot analysis in MG63 and Saos-2 cells after treatment with sarsasapogenin. *P<0.05. CSF1R, colony-stimulating factor-1 receptor.

Article Snippet: MG63 osteosarcoma cells were purchased from Procell Life Science Technology Co., Ltd. (cat. no. #CL-0157; http://www.procell.com.cn/p/mg-63-cl-0157-72575 ).

Techniques: Binding Assay, Expressing, Western Blot